BioPhoretics

Isoelectric focusing is also known as electrofocusing, is a technique for separating different molecules with their isoelectric point differences. This is a type of zone electrophoresis that is usually done on proteins in the gel that utilizes the fact that the overall charge on the molecule of interest is the function of the pH of its surroundings. Biophoretics Isoelectric Focusing Gels effectively make pH gradients so separate proteins are following their unique pi. This gel can be used to determine PI or detect small changes in protein due to deaminations, phosphorization, or glycosylation. They can also complete various proteins with similar sizes that cannot be solved on standard SDS gel pages. Biophoretics Isoelectric Focusing Gels are excellent for native, non-denaturing applications using soluble proteins. All IEF Gel is provided at 1,0 mm thickness and 5% acrylamide concentration. IEF Gels provide: 1.      Clear and sharp bands to facilitate identificatio...

DEAE-cellulose-52 (ion-exchange chromatography) of BSP (Bamboo shoot polysaccharide)! BSP1 eluted with water; BSP2 eluted with 0.05 M NaCl; BSP3 eluted with 0.1 M NaCl; BSP4 eluted with 0.2 M NaCl; BSP5 eluted with 0.5 M NaCl. The early excision products from irradiated E. coli DNA, isolated by DEAE chromatography, include oligonucleotides of nucleotides in length between 7 and 8. The isolation of these fragments by DEAE paper chromatography and their subsequent digestion by venom phosphodiesterase (which initiates hydrolysis at the 3' terminus) results in the release of those nucleotides 3' to the dimer, since this exonuclease will not proceed beyond the dimer. Exonucleolytic digestion by bovine spleen phosphodiesterase, which initiates hydrolysis at the 5′ terminus, releases those nucleotides located 5′ to the dimer. These data show that the majority of the nucleotides are located 3' to the dimer, and very few, if any, nucleotides exist on the 5′ incision side of the py...