What should you know about Deae Cellulose 52?
DEAE-cellulose-52 (ion-exchange chromatography) of BSP (Bamboo shoot polysaccharide)! BSP1 eluted with water; BSP2 eluted with 0.05 M NaCl; BSP3 eluted with 0.1 M NaCl; BSP4 eluted with 0.2 M NaCl; BSP5 eluted with 0.5 M NaCl. The early excision products from irradiated E. coli DNA, isolated by DEAE chromatography, include oligonucleotides of nucleotides in length between 7 and 8. The isolation of these fragments by DEAE paper chromatography and their subsequent digestion by venom phosphodiesterase (which initiates hydrolysis at the 3' terminus) results in the release of those nucleotides 3' to the dimer, since this exonuclease will not proceed beyond the dimer. Exonucleolytic digestion by bovine spleen phosphodiesterase, which initiates hydrolysis at the 5′ terminus, releases those nucleotides located 5′ to the dimer. These data show that the majority of the nucleotides are located 3' to the dimer, and very few, if any, nucleotides exist on the 5′ incision side of the py...